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OSU CCC Immunology Program Newsletter - September 2008 Dear Program Members, OSU CCC IMMUNOLOGY DISTINGUISHED LECTURESHIP SHARED RESOURCES OF THE OSU CCC FEATURE: The OSUCCC is proud to welcome SOLiD technology, from Applied Biosystems, Inc., to the Microarray Shared Resource. The SOLiD system is a dynamic short-read next-generation genomic analyzer which enables massively parallel sequencing of clonally-amplified DNA fragments linked to beads. SOLiD (Sequencing by Oligo Ligation Detection) sequencing methodology is based on sequential ligation with dye-labeled oligonucleotides. Genomic DNA, cDNA, RNA (and other nucleic acids) can be analyzed (sequenced or re-sequenced) for mutations, SNPs, copy number, CHiP, methylation, RNA and small RNA expression by analyzing not only the primary sequence data but also by quantitating the number of tags (beads). A major feature of this system is the two-base encoding which provides unmatched sequence accuracy compared to other platforms. SOLiD is scalable, ultra-high throughput, and has enough application flexibility to match your gene expression and genome-wide coverage needs. Depending on library type and methodology an average run yields between 6 and 15 Gb of mappable data. The instrument is located in the Microarray Shared Resource Laboratory in room 240, in the BRT. The instrument will soon be available for users. For more details or additional information... Contact: OSU CCC Immunology Program Members are encouraged to contact Dr. Gregory Lesinski with comments, suggestions or other news that can be included in future newsletters. Contact:
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