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RNA/DNA Extraction Submission Form

RNA/DNA Extraction Sample sheet

 

RNA/DNA Extraction

Please contact nucleicacid@osumc.edu or call (614) 688-5810.

Traditionally, nucleic acid extraction has been a highly repetitive and labor-intensive procedure requiring a significant time investment, and often involving the use of toxic organic chemicals. Applied Biosystems' PRISM 6100 Nucleic Acid PrepStation isolates and purifies nucleic acids, including total RNA and genomic DNA from a variety of biological samples. The device works based on an integrated vacuum system. Automated isolation of ultra pure nucleic acids, RNA and/or DNA, reduces costs and increases the quality of data for the specific application such as sequencing, genotyping, real time PCR, or microarray.

Please consult with the staff of the nucleic acid shared resource to optimize your specific needs. There are different protocols for the isolation of nucleic acids from different sources such as whole animal tissue or plant tissue.

These different protocols include steps that have to be tested and optimized such as digestion, homogenization, pre-filtration, and precipitation.

The RNA/DNA Extraction Core provides the following nucleic acid purifications

• Isolation of Total RNA from whole blood, plant and animal tissue, cultured cells (yield per well >200ug, dependent on sample type).
• Isolation of genomic DNA from mice tails and tissue, fresh and frozen blood, tissue culture cells, and buccal swabs (expected yield is 200ng to 5ug per swab).
• Isolation of RNA & DNA samples from a variety of biological samples (homogenized animal and plant tissue).

Special applications include

• Isolation of viral RNA from serum, plasma, bone marrow or CSF.
• Isolation of bacterial RNA from both gram negative and positive bacteria and cyanobacteria from culture.
• Isolation of high quality RNA from bacteria obtained from urine samples.
• Isolation of total RNA from formalin fixed paraffin embedded tissue samples and partially purified RNA samples from other purification techniques as well as yeast.
• Purification of cRNA from in-vitro transcription reactions for microarray experiments.

Guidelines:

1. In order to get a price quote for your project please fill out the Submission Form along with an explanation of your project.
2. Once we receive the project details, our lab personnel will email you the protocol along with a price estimate for your samples.
3. Email the approved price estimate to nucleicacid@osumc.edu .
4. Submit samples to 440 Wiseman Hall between the hours of 8:30 am and 5:00 pm.
5. Samples of mice tails should be 0.5-1.0 cm in length and clipped into several pieces if possible and placed into a labeled sterile 1.5 ml tube. The labels should correspond to those on the Sample Sheet submitted with the samples.
6. Frozen samples at -20°C or -80°C may be delivered between 8:30 am and 5 pm, Monday through Friday. We will inform you via email when your samples are ready for pick up.
7. Approval of charge authorization sheets must be emailed before the samples will be processed.