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Imaging

The function of the imaging systems is to simplify, automate, and accelerate DNA, RNA and protein analysis experiments.

The Typhoon and the Personal Densitometer are located in the hallway in front of BRT 236 with unlimited access after hours and weekends. There is no need for a reservation, just walk in. Phosphor screens, 20 x 25 cm, including cassettes, are available.

Typhoon 9410 Imager Applications

Non-fluorescence applications
Isotopic imaging
Chemiluminescence

Fluorescence applications
Blot detection
Gel stains
Solution stains
Covalent labels
Natural fluorescence

The Molecular Dynamics Typhoon 9410 Variable Mode Imager combines four-color fluorescence labeling techniques with storage phosphor autoradiography technology.

Five different scanning modes can be selected

• storage phosphor autoradiography
• direct green (532 nm) excited fluorescence; double frequency SYAG laser
• direct red (633 nm) excited fluorescence; Helium-Neon laser
• direct blue (457 nm, 488 nm) excited fluorescence; Argon ion laser
• chemiluminescence

The Typhoon scans mounted and unmounted storage phosphor screens, plus gels and blots up to 35 x 43 cm as well as microarray slides. It includes Typhoon scan control and ImageQuant analysis software v5.2 for Windows operating systems.

The Typhoon handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, macro- and microarrays, tissue sections, radio-TLC and X-Ray diffraction. An automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample.

Emission filter type	range nm	fluorochromes
520nm bandpass (520BP40)	500-540	Cy2,ECL Plus, fluorescin (blue)
555nm bandpass (555BP20)	545-565	R6G, HEX
580nm bandpass (580BP30)	565-595	TAMRA, Cy3
610nm bandpass (610BP30)	595-625	ROX, ethidium bromide
670nm bandpass (670BP30)	655-685	Cy5
526nm short-pass (526SP)	~526	fluorescin (green)
560nm long-pass (560LP)	~560	TRITC

Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.

Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.

Personal Densitometer SI

The Personal Densitometer SI uses a laser scanner (1 mW Helium-Neon laser, Wavelength: 632.8nm) to quantify:

• stained electrophoresis gels, up to 5 mm thick
• blotting membranes
• TLC plates as large as 20 x 25 cm and up to 3 mm thick
• films exposed by chemiluminescence or radiochemicals

It is a highly sensitive laser-based transmission densitometer, with a linear range from 0.1-3.5 OD, and a high-efficiency light collection for accurate quantification.

The Personal Densitometer SI includes scan control and ImageQuant analysis software v5.2 for Windows operating systems.